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fatty acid oxidation fao capacity  (Elabscience Biotechnology)


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    Elabscience Biotechnology fatty acid oxidation fao capacity
    Progesterone drives PPARγ-mediated fatty <t>acid</t> <t>oxidation</t> and mitophagy activation. (A) Expression of genes in the PPAR signaling pathway. The heatmap shows the RNA-seq data (rows: z scores; columns: samples). (B) GSEA of PPAR signaling. ETX coculture vs. ETX alone (NES = 1.69, FDR = 0.086). (C) GSEA of mitophagy. ETX coculture vs. ETX alone (NES = 1.53, FDR = 0.131). (D) Fatty acid oxidation <t>(FAO)</t> capacity in co-cultures. The levels of FAO were measured by ELISA in ETX-treated HSCs, co-cultures with ETX, and co-cultures with ETX plus GW9662. Data are presented as means ± SEMs; n = 3; ** P < 0.01 (one-way ANOVA followed by Tukey’s test). (E) GSEA of NRF2-ARE signaling (NES = 1.8, FDR = 0.05). (F) Western blots showing the levels of PPARγ, CPT1A, and PGC1α in cocultures ± ETX/RU486 (RU486, 2 μM). (G) Allopregnanolone levels in different groups. The concentrations of allopregnanolone were measured by ELISA in control, ETX-treated, and ETX with Dutasteride groups. Data are presented as means ± SEMs; n = 3; ** P < 0.01, *** P < 0.001 (one-way ANOVA followed by Tukey’s test). (H) Western blots showing the levels of PPARγ. (I) Densitometry of the blots in (F) . The data are presented as means ± SEMs; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Student’s t- test, one-way ANOVA followed by Tukey’s test). (J) Flow cytometry analysis of CD68 + CD206 + cells in cocultures. i. Ctrl (Etifoxine); ii. cocultures + ETX; iii. cocultures + ETX + GW9662 (GW9662; 10 μM). Data are presented as means ± SEMs; n = 3; *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Tukey’s test).
    Fatty Acid Oxidation Fao Capacity, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fatty+acid+oxidation+fao+capacity/Fatty+Acid+Oxidation+(FAO)+Colorimetric+Assay+Kit/pmc13060035-77-1-13
    Average 94 stars, based on 52 article reviews
    fatty acid oxidation fao capacity - by Bioz Stars, 2026-10
    94/100 stars

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    1) Product Images from "Etifoxine drives macrophage M2 polarization via Schwann cell-derived progesterone activation of PPARγ to accelerate peripheral nerve repair"

    Article Title: Etifoxine drives macrophage M2 polarization via Schwann cell-derived progesterone activation of PPARγ to accelerate peripheral nerve repair

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2026.1789450

    Progesterone drives PPARγ-mediated fatty acid oxidation and mitophagy activation. (A) Expression of genes in the PPAR signaling pathway. The heatmap shows the RNA-seq data (rows: z scores; columns: samples). (B) GSEA of PPAR signaling. ETX coculture vs. ETX alone (NES = 1.69, FDR = 0.086). (C) GSEA of mitophagy. ETX coculture vs. ETX alone (NES = 1.53, FDR = 0.131). (D) Fatty acid oxidation (FAO) capacity in co-cultures. The levels of FAO were measured by ELISA in ETX-treated HSCs, co-cultures with ETX, and co-cultures with ETX plus GW9662. Data are presented as means ± SEMs; n = 3; ** P < 0.01 (one-way ANOVA followed by Tukey’s test). (E) GSEA of NRF2-ARE signaling (NES = 1.8, FDR = 0.05). (F) Western blots showing the levels of PPARγ, CPT1A, and PGC1α in cocultures ± ETX/RU486 (RU486, 2 μM). (G) Allopregnanolone levels in different groups. The concentrations of allopregnanolone were measured by ELISA in control, ETX-treated, and ETX with Dutasteride groups. Data are presented as means ± SEMs; n = 3; ** P < 0.01, *** P < 0.001 (one-way ANOVA followed by Tukey’s test). (H) Western blots showing the levels of PPARγ. (I) Densitometry of the blots in (F) . The data are presented as means ± SEMs; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Student’s t- test, one-way ANOVA followed by Tukey’s test). (J) Flow cytometry analysis of CD68 + CD206 + cells in cocultures. i. Ctrl (Etifoxine); ii. cocultures + ETX; iii. cocultures + ETX + GW9662 (GW9662; 10 μM). Data are presented as means ± SEMs; n = 3; *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Tukey’s test).
    Figure Legend Snippet: Progesterone drives PPARγ-mediated fatty acid oxidation and mitophagy activation. (A) Expression of genes in the PPAR signaling pathway. The heatmap shows the RNA-seq data (rows: z scores; columns: samples). (B) GSEA of PPAR signaling. ETX coculture vs. ETX alone (NES = 1.69, FDR = 0.086). (C) GSEA of mitophagy. ETX coculture vs. ETX alone (NES = 1.53, FDR = 0.131). (D) Fatty acid oxidation (FAO) capacity in co-cultures. The levels of FAO were measured by ELISA in ETX-treated HSCs, co-cultures with ETX, and co-cultures with ETX plus GW9662. Data are presented as means ± SEMs; n = 3; ** P < 0.01 (one-way ANOVA followed by Tukey’s test). (E) GSEA of NRF2-ARE signaling (NES = 1.8, FDR = 0.05). (F) Western blots showing the levels of PPARγ, CPT1A, and PGC1α in cocultures ± ETX/RU486 (RU486, 2 μM). (G) Allopregnanolone levels in different groups. The concentrations of allopregnanolone were measured by ELISA in control, ETX-treated, and ETX with Dutasteride groups. Data are presented as means ± SEMs; n = 3; ** P < 0.01, *** P < 0.001 (one-way ANOVA followed by Tukey’s test). (H) Western blots showing the levels of PPARγ. (I) Densitometry of the blots in (F) . The data are presented as means ± SEMs; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Student’s t- test, one-way ANOVA followed by Tukey’s test). (J) Flow cytometry analysis of CD68 + CD206 + cells in cocultures. i. Ctrl (Etifoxine); ii. cocultures + ETX; iii. cocultures + ETX + GW9662 (GW9662; 10 μM). Data are presented as means ± SEMs; n = 3; *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Tukey’s test).

    Techniques Used: Activation Assay, Expressing, RNA Sequencing, Enzyme-linked Immunosorbent Assay, Western Blot, Control, Flow Cytometry

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    Colorimetric Assay:

    Article Title: Etifoxine drives macrophage M2 polarization via Schwann cell-derived progesterone activation of PPARγ to accelerate peripheral nerve repair
    Article Snippet: The results of triplicate experiments were analyzed using ImageJ ( RRID:SCR_003070 ) for grayscale quantification. .. The fatty acid oxidation (FAO) capacity was quantified using a colorimetric assay kit (Elabscience ® #E-BC-K784-M). ..



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    Elabscience Biotechnology fatty acid oxidation fao capacity
    Progesterone drives PPARγ-mediated fatty <t>acid</t> <t>oxidation</t> and mitophagy activation. (A) Expression of genes in the PPAR signaling pathway. The heatmap shows the RNA-seq data (rows: z scores; columns: samples). (B) GSEA of PPAR signaling. ETX coculture vs. ETX alone (NES = 1.69, FDR = 0.086). (C) GSEA of mitophagy. ETX coculture vs. ETX alone (NES = 1.53, FDR = 0.131). (D) Fatty acid oxidation <t>(FAO)</t> capacity in co-cultures. The levels of FAO were measured by ELISA in ETX-treated HSCs, co-cultures with ETX, and co-cultures with ETX plus GW9662. Data are presented as means ± SEMs; n = 3; ** P < 0.01 (one-way ANOVA followed by Tukey’s test). (E) GSEA of NRF2-ARE signaling (NES = 1.8, FDR = 0.05). (F) Western blots showing the levels of PPARγ, CPT1A, and PGC1α in cocultures ± ETX/RU486 (RU486, 2 μM). (G) Allopregnanolone levels in different groups. The concentrations of allopregnanolone were measured by ELISA in control, ETX-treated, and ETX with Dutasteride groups. Data are presented as means ± SEMs; n = 3; ** P < 0.01, *** P < 0.001 (one-way ANOVA followed by Tukey’s test). (H) Western blots showing the levels of PPARγ. (I) Densitometry of the blots in (F) . The data are presented as means ± SEMs; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Student’s t- test, one-way ANOVA followed by Tukey’s test). (J) Flow cytometry analysis of CD68 + CD206 + cells in cocultures. i. Ctrl (Etifoxine); ii. cocultures + ETX; iii. cocultures + ETX + GW9662 (GW9662; 10 μM). Data are presented as means ± SEMs; n = 3; *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Tukey’s test).
    Fatty Acid Oxidation Fao Capacity, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fatty+acid+oxidation+fao+capacity/Fatty+Acid+Oxidation+(FAO)+Colorimetric+Assay+Kit/pmc13060035-77-1-13
    Average 94 stars, based on 1 article reviews
    fatty acid oxidation fao capacity - by Bioz Stars, 2026-10
    94/100 stars
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    Progesterone drives PPARγ-mediated fatty acid oxidation and mitophagy activation. (A) Expression of genes in the PPAR signaling pathway. The heatmap shows the RNA-seq data (rows: z scores; columns: samples). (B) GSEA of PPAR signaling. ETX coculture vs. ETX alone (NES = 1.69, FDR = 0.086). (C) GSEA of mitophagy. ETX coculture vs. ETX alone (NES = 1.53, FDR = 0.131). (D) Fatty acid oxidation (FAO) capacity in co-cultures. The levels of FAO were measured by ELISA in ETX-treated HSCs, co-cultures with ETX, and co-cultures with ETX plus GW9662. Data are presented as means ± SEMs; n = 3; ** P < 0.01 (one-way ANOVA followed by Tukey’s test). (E) GSEA of NRF2-ARE signaling (NES = 1.8, FDR = 0.05). (F) Western blots showing the levels of PPARγ, CPT1A, and PGC1α in cocultures ± ETX/RU486 (RU486, 2 μM). (G) Allopregnanolone levels in different groups. The concentrations of allopregnanolone were measured by ELISA in control, ETX-treated, and ETX with Dutasteride groups. Data are presented as means ± SEMs; n = 3; ** P < 0.01, *** P < 0.001 (one-way ANOVA followed by Tukey’s test). (H) Western blots showing the levels of PPARγ. (I) Densitometry of the blots in (F) . The data are presented as means ± SEMs; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Student’s t- test, one-way ANOVA followed by Tukey’s test). (J) Flow cytometry analysis of CD68 + CD206 + cells in cocultures. i. Ctrl (Etifoxine); ii. cocultures + ETX; iii. cocultures + ETX + GW9662 (GW9662; 10 μM). Data are presented as means ± SEMs; n = 3; *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Tukey’s test).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Etifoxine drives macrophage M2 polarization via Schwann cell-derived progesterone activation of PPARγ to accelerate peripheral nerve repair

    doi: 10.3389/fncel.2026.1789450

    Figure Lengend Snippet: Progesterone drives PPARγ-mediated fatty acid oxidation and mitophagy activation. (A) Expression of genes in the PPAR signaling pathway. The heatmap shows the RNA-seq data (rows: z scores; columns: samples). (B) GSEA of PPAR signaling. ETX coculture vs. ETX alone (NES = 1.69, FDR = 0.086). (C) GSEA of mitophagy. ETX coculture vs. ETX alone (NES = 1.53, FDR = 0.131). (D) Fatty acid oxidation (FAO) capacity in co-cultures. The levels of FAO were measured by ELISA in ETX-treated HSCs, co-cultures with ETX, and co-cultures with ETX plus GW9662. Data are presented as means ± SEMs; n = 3; ** P < 0.01 (one-way ANOVA followed by Tukey’s test). (E) GSEA of NRF2-ARE signaling (NES = 1.8, FDR = 0.05). (F) Western blots showing the levels of PPARγ, CPT1A, and PGC1α in cocultures ± ETX/RU486 (RU486, 2 μM). (G) Allopregnanolone levels in different groups. The concentrations of allopregnanolone were measured by ELISA in control, ETX-treated, and ETX with Dutasteride groups. Data are presented as means ± SEMs; n = 3; ** P < 0.01, *** P < 0.001 (one-way ANOVA followed by Tukey’s test). (H) Western blots showing the levels of PPARγ. (I) Densitometry of the blots in (F) . The data are presented as means ± SEMs; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Student’s t- test, one-way ANOVA followed by Tukey’s test). (J) Flow cytometry analysis of CD68 + CD206 + cells in cocultures. i. Ctrl (Etifoxine); ii. cocultures + ETX; iii. cocultures + ETX + GW9662 (GW9662; 10 μM). Data are presented as means ± SEMs; n = 3; *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Tukey’s test).

    Article Snippet: The fatty acid oxidation (FAO) capacity was quantified using a colorimetric assay kit (Elabscience ® #E-BC-K784-M).

    Techniques: Activation Assay, Expressing, RNA Sequencing, Enzyme-linked Immunosorbent Assay, Western Blot, Control, Flow Cytometry